| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Alkaline gel electrophoresis assay to detect DNA strand breaks and repair mechanisms in Escherichia coli
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| Autores: |
Mattos,José Carlos Pelielo de
Motta,Ellen Serri da
Oliveira,Márcia Betania Nunes de
Dantas,Flávio José da Silva
Araujo,Adriano Caldeira de
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| Data: |
2008-12-01
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| Ano: |
2008
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| Palavras-chave: |
Alkaline gel electrophoresis
Escherichia coli
Base excision repair
Stannous chloride
DNA strand breaks
Reactive oxygen species
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| Resumo: |
Reactive oxygen species (ROS) can induce lesions in different cellular targets, including DNA. Stannous chloride (SnCl2) is a ROS generator, leading to lethality in Escherichia coli (E. coli), with the base excision repair (BER) mechanism playing a role in this process. Many techniques have been developed to detect genotoxicity, as comet assay, in eukaryotic cells, and plasmid DNA agarose gel electrophoresis. In this study, an adaptation of the alkaline gel electrophoresis method was carried out to ascertain the induction of strand breaks by SnCl2 in bacterial DNA, from E. coli BER mutants, and its repair pathway. Results obtained show that SnCl2 was able to induce DNA strand breaks in all strains tested. Moreover, endonuclease IV and exonuclease III play a role in DNA repair. On the whole, data has shown that the alkaline gel electrophoresis assay could be used both for studying DNA strand breaks induction and for associated repair mechanisms.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000700020
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132008000700020
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.51 n.spe 2008
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| Direitos: |
info:eu-repo/semantics/openAccess
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